
Halophilic Bacteria Selective Agar – 250g | Vibrio parahaemolyticus Purification Medium
Halophilic Bacteria Selective Agar is a specialized medium for purifying Vibrio parahaemolyticus from mixed cultures and environmental samples.
Primary Application:
✔️ Purification of halophilic vibrios
✔️ Post-enrichment isolation
✔️ Seafood safety testing
Formula (Per Liter):
– Peptone: 20.0g
– Sodium Chloride: 40.0g
– Crystal Violet: 5.0mg
– Agar: 17.0g
pH 8.7 ± 0.1 (25°C)
Protocol:
1️⃣ Suspend 77.0g in 1L water
2️⃣ Boil ≥1 minute
3️⃣ Autoclave at 121°C for 15 minutes
4️⃣ Swirl before pouring plates
Key Features:
– 4% NaCl for halophilic selection
– Alkaline pH suppression
– Ready-to-use powder
– 3-year stability