
Glucose Semisolid Agar for Acid/Gas Detection
Dual-Purpose Medium for Glucose Fermentation & Motility Testing
This semisolid agar with bromcresol purple indicator simultaneously detects acid production (color change) and gas formation (fissures) in bacterial glucose metabolism studies.
Key Features:
Low agar concentration (3g/L) for optimal motility observation
Pre-mixed glucose (10g/L) for immediate fermentation
Room temperature stable (3-year shelf life)
Clear purple-to-yellow transition at pH shift
Technical Specifications:
▶ Formula (per liter):
Peptone 10.0g | Beef Extract 3.0g | NaCl 5.0g
Dextrose 10.0g | BCP 0.016g | Agar 3.0g
▶ pH: 7.4±0.2 (25°C)
▶ Preparation: 31g/L → boil → sterilize at 121°C/15min → solidify upright
Diagnostic Applications:
Differentiation of Enterobacteriaceae
Detection of non-fermenting bacteria
Microbial motility characterization