Giolitti-Cantoni Broth Base | GC Broth Medium for Staphylococci MPN Counting

Giolitti-Cantoni Broth Base for Staphylococcus MPN Detection | Dehydrated Culture Medium 250g

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Giolitti-Cantoni Broth Base is a dehydrated microbiological culture medium designed for the Most Probable Number (MPN) enumeration of Staphylococcus species in laboratory microbiology testing.
The medium requires the addition of 1% potassium tellurite solution for selective detection.

This powdered medium provides reliable performance, consistent formulation, and convenient preparation for microbiological quality control and research laboratories.

Packaging: 250g per bottle
Storage: Room temperature
Shelf life: 3 years

Giolitti-Cantoni Broth Base

Giolitti-Cantoni Broth Base is a dehydrated microbiological culture medium used for the detection and enumeration of Staphylococcus using the Most Probable Number (MPN) method.

The formulation supports the growth of target organisms while improving selectivity when supplemented with potassium tellurite solution.

It is widely used in microbiology laboratories for food safety testing, hygiene monitoring, and microbial research where reliable Staphylococcus enumeration is required.

Key Features

  • Designed for Staphylococcus MPN enumeration
  • Reliable microbiological culture medium
  • Dehydrated powder format for convenient storage
  • Consistent formulation for laboratory testing
  • Suitable for microbiology research and quality control
  • Easy preparation and sterilization

Medium Composition (per liter)

Tryptone ……………………………. 10.0 g
Beef Extract ………………………… 5.0 g
Yeast Extract ……………………….. 5.0 g
D-Mannitol …………………………… 20.0 g
Sodium Chloride …………………… 5.0 g
Lithium Chloride ……………………. 5.0 g
Glycine ………………………………. 1.2 g
Sodium Pyruvate ……………………. 3.0 g

pH: 6.9 ± 0.2 (25°C)


Preparation Instructions

  1. Suspend 54.2 g of the medium in 1 liter of distilled or deionized water.
  2. Sterilize by autoclaving at 121°C for 15 minutes.
  3. Cool the medium to approximately 50°C.
  4. Aseptically add 1.05 ml of 1% potassium tellurite solution and mix well.
  5. Dispense 19 ml per sterile test tube (20 × 200 mm) for use.

Storage

Store tightly sealed in a cool, dry place at room temperature.
Shelf life: 3 years.

ParameterSpecification
Product NameGiolitti-Cantoni Broth Base
FormatDehydrated Powder
Net Content250 g
StorageRoom Temperature
Shelf Life3 Years
ApplicationStaphylococcus MPN Enumeration

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