Geobacter metallireducens Medium (ATCC Medium 1768) | Basal Medium for Geobacter Cultivation

Geobacter metallireducens Medium (ATCC Medium 1768) is a basal medium designed for the cultivation of Geobacter metallireducens. This medium requires supplementation with ferric citrate solution and sodium acetate solution for optimal growth under anaerobic conditions.

Specifications:

• Format: Powder, 500 g/bottle

• Storage: Room temperature

• Shelf Life: 3 years

Formula (per liter):

• Wolfe’s Vitamin Solution – 10 ml

• Wolfe’s Mineral Solution – 10 ml

• NaHCO₃ – 2.5 g

• NH₄Cl – 0.25 g

• NaH₂PO₄·H₂O – 0.6 g

• KCl – 0.1 g

Preparation Instructions:

1. Dissolve 3.51 g of medium in 950 ml of distilled or deionized water, boil gently until fully dissolved.

2. Dispense into suitable containers.

3. For every 95 ml of medium, aseptically add 1 vial of Ferric Citrate solution and 1 vial of Sodium Acetate solution.

4. Cool to 25℃.

5. Adjust pH to 5.0 with sterile 10N NaOH and maintain stability.

6. Sparge with an N₂/CO₂ (80:20) gas mixture to create anaerobic conditions.

7. Adjust final pH to 6.8–7.0.

8. Sterilize by autoclaving at 121℃ for 15 minutes.