
Gentamicin Agar Base – 250g | Selective Medium for Vibrio cholerae
Gentamicin Agar Base is a selective medium for isolating Vibrio cholerae from clinical and environmental samples.
Applications:
✔️ Primary isolation in cholera outbreaks
✔️ Water and food safety testing
✔️ Clinical specimen processing
Formula (Per Liter):
– Peptone: 10.0g
– Beef Extract: 3.0g
– Sodium Sulfite: 3.0g
– Sodium Citrate: 10.0g
– Sodium Chloride: 5.0g
– Sucrose: 10.0g
– Agar: 15.0g
pH 8.4 ± 0.2 (25°C)
Protocol:
1️⃣ Suspend 56.0g in 1L water
2️⃣ Boil 1-2 minutes
3️⃣ Cool to 50-55°C
4️⃣ Add 1 Gentamicin Supplement per 100ml
5️⃣ Mix → Pour plates
Key Features:
– Alkaline pH selectivity
– Ready-to-use powder
– 3-year shelf stability