GC Agar Base – 250g for Selective Cultivation of Pathogenic Neisseria

GC Agar Base is a specialized culture medium designed for the selective isolation and cultivation of pathogenic Neisseria species, including Neisseria gonorrhoeae. It supports optimal bacterial growth while suppressing contaminating flora, ideal for clinical microbiology, diagnostics, and laboratory research.

Product Information

• Product Name: GC Agar Base

• Pack Size: 250g per bottle

• Form: Dehydrated powder

• Storage: Room temperature

• Shelf Life: 3 years

Applications

• Isolation and cultivation of pathogenic Neisseria

• Clinical specimen culture

• Microbiological research and diagnostics

Medium Composition (per liter)

• Enzymatic Digest of Casein: 7.5 g

• Enzymatic Digest of Animal Tissue: 7.5 g

• Corn Starch: 1.0 g

• Dipotassium Phosphate: 4.0 g

• Monopotassium Phosphate: 1.0 g

• Sodium Chloride: 5.0 g

• Agar: 10.0 g

• pH: 7.2 ± 0.2 (25℃)

Preparation Instructions

1. Dissolve 3.6 g of GC Agar Base in 50 ml distilled or deionized water.

2. Sterilize by autoclaving at 121℃ for 15 minutes, then cool to 50–55℃.

3. Dissolve 1 vial of sterile hemoglobin powder in 50 ml sterile water (with glass beads if needed) and preheat to ~50℃.

4. Add hemoglobin solution along the wall of the flask to the sterilized agar, mix gently.

5. Aseptically add 1 vial each of IsoVitaleX supplement and VCN selective inhibitor per 100 ml medium, mix gently.

6. Pour plates quickly to prevent agar solidification.