Gauze’s Synthetic Broth Medium is a microbiological culture medium designed for the cultivation of actinomycetes and related microorganisms. The formulation provides a balanced nutrient composition that supports microbial metabolism and growth in laboratory culture systems.
This microbiological broth medium is supplied as a dehydrated powder, allowing convenient preparation in microbiology laboratories. After dissolution and sterilization, the medium forms a liquid culture environment suitable for actinomycetes and Streptomyces cultivation.
The medium is widely used in environmental microbiology laboratories, soil microorganism studies, and academic research involving actinomycetes and Streptomyces species.
For selective culture applications, potassium dichromate solution may optionally be added after sterilization to increase inhibitory selectivity when required.
Key Features
- Designed for actinomycetes cultivation
- Suitable for Streptomyces culture
- Dehydrated microbiological broth culture medium
- Easy preparation and sterilization
- Suitable for liquid microbial culture
- 250 g laboratory packaging
- Stable formulation with 3-year shelf life
Applications
- Cultivation of actinomycetes
- Streptomyces microbial studies
- Soil microorganism culture
- Environmental microbiology research
- Academic microbiology laboratories
Medium Composition
Composition (per liter)
Soluble Starch — 20.0 g
Potassium Nitrate — 1.0 g
Dipotassium Phosphate — 0.5 g
Magnesium Sulfate — 0.5 g
Ferrous Sulfate — 0.01 g
Sodium Chloride — 0.5 g
Final pH: 7.3 ± 0.1 (25°C)
Directions for Use
- Suspend 22.5 g of the medium in 1 liter of distilled or deionized water.
- Heat to boiling and dissolve for 1 minute.
- Adjust the final volume to 1000 ml with water.
- Dispense into appropriate containers.
- Sterilize by autoclaving at 121°C for 15 minutes and allow to cool.
If selective inhibition is required, 1 ml of 3% potassium dichromate solution may be added to every 300 ml of medium after sterilization. Mix thoroughly before use.
Product Specifications
| Item | Description |
|---|---|
| Product Name | Gauze’s Synthetic Broth Medium |
| Medium Type | Broth Culture Medium |
| Format | Powder |
| Package | 250 g / bottle |
| Storage | Room temperature |
| Shelf Life | 3 years |
| Application | Actinomycetes cultivation |