Gauze’s Synthetic Agar Medium, Modified 250g for Actinobacteria Cultivation

Gauze’s Synthetic Agar Medium, Modified, is designed specifically for the cultivation of Actinobacteria. It is a modification of the original Gauze’s Synthetic Agar Medium, widely used for research and biotechnological applications involving Actinobacteria species, including actinomycetes.

Composition per liter:

• Soluble Starch: 20.0 g

• Potassium Nitrate (KNO3): 1.0 g

• Dipotassium Phosphate (K2HPO4): 0.5 g

• Magnesium Sulfate (MgSO4): 0.5 g

• Sodium Chloride (NaCl): 0.5 g

• Agar: 15.0 g

• Final pH: 7.3 ± 0.1 (25℃)

Preparation:
Weigh 37.5 g of the medium and dissolve it in 1 L of distilled water or deionized water. Boil for 1 minute to dissolve completely, then add water to make up to 1000 ml. Dispense into containers and sterilize at 121℃ for 15 minutes. Shake the medium after sterilization to prevent agar from settling at the bottom. If needed, cool to 55℃, add 1 ml of 3% Potassium Chromate solution per 300 ml of medium to enhance antimicrobial properties, mix thoroughly, and pour into sterile plates.

Packaging: 250 g per bottle
Storage: Ambient temperature
Shelf life: 3 years