Gauze’s Agar Medium No.2 Modified for Actinomycetes Isolation | Microbiological Agar Medium Powder 250g

Gauze’s Agar Medium No.2, Modified is a microbiological culture medium formulated for the isolation and cultivation of actinomycetes and related microorganisms. The formulation contains essential nutrients and vitamins that support microbial metabolism and colony development in laboratory environments.

This microbiological agar medium is supplied as a dehydrated powder, allowing convenient preparation in laboratories. After dissolution and sterilization, the medium forms a stable agar environment suitable for actinomycete isolation and culture.

The medium is commonly used in microbiology laboratories, environmental microbial studies, soil microorganism research, and academic actinomycete studies.

Key Features

• Designed for actinomycetes isolation and cultivation
• High-quality microbiological agar culture medium
• Supplied as dehydrated powder medium
• Suitable for Streptomyces and related microorganisms
• Easy preparation and sterilization
• 250 g laboratory packaging
• Stable formulation with 3-year shelf life

Applications

• Isolation of actinomycetes
• Streptomyces cultivation and research
• Environmental microbiology studies
• Soil microorganism isolation
• Academic microbiology laboratories

Medium Composition

Composition (per liter)

Sodium Chloride (NaCl) — 0.5 g
Tryptone — 0.3 g
Glucose — 1.0 g
Peptone — 0.5 g
Vitamin B1 — 0.5 mg
Vitamin B2 — 0.5 mg
Vitamin B6 — 0.5 mg
Nicotinic Acid — 0.5 mg
Inositol — 0.5 mg
Pantothenic Acid — 0.5 mg
Biotin — 0.25 mg
p-Aminobenzoic Acid — 0.5 mg
Agar — 20.0 g

Final pH: 7.2 ± 0.2 (25°C)

Directions for Use

1. Suspend 22.3 g of the medium in 1 liter of distilled or deionized water.
2. Heat and boil for at least 1 minute until completely dissolved.
3. Dispense into suitable containers.
4. Sterilize by autoclaving at 121°C for 15 minutes.
5. Allow the medium to cool before use or pour into petri dishes if agar plates are required.

Product Specifications