Folic Acid Assay Medium – Culture Medium for Folate Determination, 250g

Folic Acid Assay Medium is a specialized culture medium formulated for the quantitative determination of Folic Acid (Vitamin B9). With carefully controlled nutrient composition, it ensures high sensitivity and reproducibility in vitamin assay applications, making it ideal for microbiology and biochemical laboratories.

Complete Formula (per liter):

• Casein Hydrolysate: 10.0 g

• Glucose: 40.0 g

• Sodium Acetate: 40.0 g

• K2HPO4: 1.0 g

• KH2PO4: 1.0 g

• DL-Tryptophan: 0.2 g

• L-Aspartic Acid: 0.6 g

• Adenine Sulfate: 10 mg

• L-Cysteine Hydrochloride: 0.5 g

• Guanine Hydrochloride: 10 mg

• Uracil: 10 mg

• Xanthine: 20 mg

• Polysorbate 80 (Tween 80): 0.1 g

• Glutathione: 5 mg

• MgSO4: 0.4 g

• NaCl: 20 mg

• FeSO4: 20 mg

• MnSO4: 15 mg

• Riboflavin: 1 mg

• p-Aminobenzoic Acid: 2 mg

• Vitamin B6 (Pyridoxine): 4 mg

• Thiamine Hydrochloride: 400 µg

• Calcium Pantothenate: 800 µg

• Niacin: 800 µg

• Biotin: 20 µg

• Final pH: 6.7 ± 0.1 at 25°C

Instructions for Use:

1. Dissolve 9.39 g of medium in 100 ml of distilled or deionized water.

2. Heat until completely dissolved, then allow to cool.

3. Dispense into tubes or flasks as required.

4. Use according to standard folic acid assay procedures.

Packaging & Storage:

• 250 g per bottle

• Shelf life: 3 years

• Store at room temperature in a cool, dry place