Fluid Thioglycollate Medium (FTM) – USP/EP Sterility Testing Medium for Aerobic/Anaerobic Cultures
Pharmaceutical-Grade Fluid Thioglycollate Medium – Gold Standard for Sterility Testing
Meet stringent pharmacopeial requirements with our ready-to-prepare Fluid Thioglycollate Medium (FTM) – the essential dual-purpose medium for detecting aerobic and anaerobic microorganisms in pharmaceutical products, medical devices, and biologics. Formulated with resazurin indicator and thioglycollate reducing agent to comply with USP <71>, EP 2.6.1, and FDA sterility testing protocols.
Critical Applications:
✔️ Pharmaceutical Sterility Testing – USP/EP-compliant batch release
✔️ Medical Device Validation – Implantable device monitoring
✔️ Biologics Safety Testing – Vaccine/cell therapy sterility assurance
✔️ Anaerobic Pathogen Recovery – Clostridium spp. detection
✔️ Environmental Monitoring – Cleanroom media fills
Oxygen Gradient Technology (per liter):
| Component | Quantity | Function |
|—————————-|———-|——————————|
| Pancreatic Casein Digest | 15.0g | Nitrogen/Peptide Source |
| Yeast Extract | 5.0g | Vitamin Complex |
| L-Cystine | 0.5g | Reducing Agent |
| Sodium Thioglycollate | 0.5g | Oxygen Scavenger* |
| Sodium Chloride | 2.5g | Osmotic Balance |
| Dextrose Anhydrous | 5.0g | Energy Source |
| Purified Agar | 0.75g | Viscosity Stabilizer |
| Resazurin | 0.001g | Oxygen Indicator** |
pH: 7.1 ± 0.2 (25°C)
Maintains anaerobic conditions in lower zone
Pink = Oxidized (O₂ present) | Colorless = Reduced (Anaerobic)
Validated Preparation Protocol:
Dissolve 29.25g in 1L purified water
Boil 1 minute with stirring
Autoclave: 121°C/15 min or 115°C/30 min
Rapid Cooling: Water bath immediately post-sterilization
