Fluid Selenite-Cystine Medium (250g) for selective enrichment of Salmonella

Fluid Selenite-Cystine Medium (SC Medium)

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Fluid Selenite-Cystine Medium (SC Medium) is a selective enrichment liquid medium designed for the enhanced recovery and enrichment of Salmonella species from food, water, pharmaceutical, and clinical-related samples.

Sodium selenite inhibits competing intestinal flora, while L-cystine reduces the toxic effect of selenite, improving the survival and growth of Salmonella. Lactose and phosphate buffer help maintain suitable osmotic balance and pH during incubation.

⚠ For laboratory research and quality control use only. Not intended for clinical diagnostic or therapeutic use.

🔬 Intended Use

  • Selective enrichment of Salmonella spp.

  • Food microbiology testing

  • Water quality analysis

  • Pharmaceutical and environmental microbiology

  • Pre-enrichment prior to plating on selective agar media

📦 Product Specifications

  • Product Name: Fluid Selenite-Cystine Medium

  • Common Name: SC Medium

  • Physical Form: Dehydrated powder

  • Package Size: 250 g / bottle

  • Storage Condition: Room temperature, dry place

  • Shelf Life: 3 years

🧬 Medium Composition (per liter)

  • Pancreatic Digest of Casein …………… 5.0 g
  • Lactose ………………………………………….. 4.0 g
  • Sodium Phosphate (Tribasic) ………. 10.0 g
  • Sodium Acid Selenite ………………….. 4.0 g
  • L-Cystine ……………………………………… 10 mg
  • Final pH: 7.0 ± 0.2 (at 25 °C)

⚗️ Preparation Instructions

  1. Suspend 23.0 g of dehydrated medium in 1,000 ml of distilled or deionized water
  2. Heat gently with stirring until completely dissolved
  3. Dispense into suitable containers
  4. Do NOT autoclave
  5. Avoid excessive heating
  6. Cool to room temperature before use

⚠ This medium should be freshly prepared and used on the same day.

🧫 Storage & Stability

  • Store dehydrated medium at room temperature, protected from moisture and light
  • Prepared medium is not stable for long-term storage
  • Do not use if precipitation, discoloration, or degradation is observed

⚠️ Limitations & Precautions

  • Autoclaving will destroy the selective properties of the medium
  • Excessive heating reduces selectivity
  • Follow appropriate biosafety and disposal procedures when handling selenite-containing media

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