Bottle of Eugon LT 100 Agar Base for cosmetic total bacteria count testing

Eugon LT 100 Agar Base – Total Bacteria Count in Cosmetics

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Our Eugon LT 100 Agar Base (also called Eugon LT100 Agar Base) is a specialized ready-to-prepare culture medium for accurate total bacteria count determination in cosmetics. It’s suitable for cosmetic testing labs, QC facilities, and research institutions. A key step: add 1 vial (1ml) of Tween 80 per 200ml of prepared agar. Each 250g bottle has a 3-year shelf life at room temperature, no refrigeration required.​

Key Features​

  • Cosmetic-Specific Use: Formulated for cosmetic bacteria count testing, compatible with cosmetic ingredients.​

  • Tween 80 Compatible: Requires 1ml Tween 80 per 200ml prepared agar for optimal performance.​

  • Easy Preparation: Simple heating-to-dissolve process; post-sterilization shaking and plate pouring for use.​

  • Normal Clumping: Slight moisture or clumping due to Octoxynol 9 is common and doesn’t affect product quality.​

  • Convenient Storage: Room-temperature (15-25℃) storage; 3-year shelf life reduces reorder frequency.​

Medium Formula (Per Liter)​

Key ingredients and proportions (per liter):​

  • Pancreatic Digest of Casein: 15.0g | Papaic Digest of Soybean Meal: 5.0g​

  • L-Cystine: 0.7g | Sodium Chloride: 4.0g | Sodium Sulfite: 0.2g​

  • Glucose: 5.5g | Egg Lecithin: 1.0g | Octoxynol 9: 1.0g​

  • Agar: 15.0g​

  • pH: 7.3±0.2 (25℃)​

Usage Instructions​

  1. Weigh 47.4g of agar base, dissolve in 1L distilled/deionized water.​

  1. Heat to gentle boil, stir until fully dissolved (no undissolved particles).​

  1. Dispense into containers; add 1ml Tween 80 per 200ml agar, mix gently.​

  1. Autoclave at 121℃ for 15 minutes to sterilize.​

  1. Shake thoroughly after sterilization, then pour into plates and set aside for use.

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