Enumeration Agar (EA) – 250g | Colony Count Media for Antimicrobial Textile Testing
Enumeration Agar (EA) is a specialized culture medium designed for precise microbial colony counting on antimicrobial-treated textiles, fabrics, and nonwoven materials in compliance with international test standards.
Key Applications:
✔️ Quantitative evaluation of antibacterial textile efficacy
✔️ Total viable count (TVC) per ISO 20743 and AATCC 100
✔️ Microbial challenge testing for textile preservatives
✔️ Quality control of functional fabric treatments
Optimized Formula Per Liter:
| Component | Quantity |
|————————-|———-|
| Dehydrated Yeast Extract| 2.5 g |
| Casein Tryptone | 5.0 g |
| Glucose | 1.0 g |
| Agar | 17.0 g |
Final pH: 7.2 ± 0.2 (25°C)
Enhanced Protocol:
1️⃣ Dissolve 25.5g powder in 1L distilled water
2️⃣ Autoclave at 121°C for 15 minutes
3️⃣ Swirl gently post-sterilization to prevent agar sedimentation
Critical Advantages:
– Textile-specific formulation: Low-nutrient profile prevents overgrowth for clear colony differentiation
– Optimal clarity: High-purity agar enables easy colony enumeration
– Standardized results: Consistent performance across textile types (cotton, polyester, blends)
– Extended shelf life: 3-year stability at ambient storage
Industry Compliance:
– Validated for ISO 20743 (JIS L 1902) quantitative testing
– Compatible with AATCC 100 assessment protocols
