Enterobacteria Enrichment Broth Mossel (250g) for intestinal bacteria enrichment culture

Enterobacteria Enrichment Broth (Mossel Method)

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Enterobacteria Enrichment Broth (Mossel) is a selective liquid culture medium designed for the enrichment of bile salt–tolerant Gram-negative enteric bacteria from food, environmental, and quality control samples.

The formulation combines bile salts and brilliant green to suppress competing Gram-positive organisms, while glucose and buffered phosphates support the growth of target enterobacteria. This medium is commonly used as a pre-enrichment or selective enrichment step prior to plating on selective agar media.

For laboratory research and quality control use only. Not intended for clinical or therapeutic applications.


🔬 Intended Use

  • Enrichment of enterobacteria

  • Selective growth of bile-resistant Gram-negative bacteria

  • Food microbiology testing

  • Environmental and hygiene monitoring


📦 Product Specifications

  • Product Name: Enterobacteria Enrichment Broth, Mossel

  • Physical Form: Dehydrated powder

  • Package Size: 250 g / bottle

  • Storage Condition: Room temperature, dry place

  • Shelf Life: 3 years

🧬 Medium Composition (per liter)

  • Pancreatic Digest of Gelatin …………………. 10.0 g
  • Disodium Phosphate, Anhydrous ………….. 6.4 g
  • Glucose, Anhydrous ………………………………. 4.5 g
  • Bile Salts …………………………………………………. 3.055 g
    (sodium deoxycholate, sodium lauryl sulfate, sodium citrate)
  • Potassium Dihydrogen Phosphate ………… 2.0 g
  • Brilliant Green …………………………………………. 0.015 g

Final pH: 7.2 ± 0.2 (at 25 °C)


⚗️ Preparation Instructions

  1. Suspend 26.0 g of dehydrated medium in 1,000 ml of distilled or deionized water
  2. Warm gently with stirring until fully dissolved
  3. Dispense into suitable containers
  4. Heat in a boiling water bath at 100 °C for 30 minutes
  5. Cool rapidly after heating
  6. Medium is ready for use

Do NOT autoclave. High-pressure sterilization is strictly prohibited.


🧫 Storage & Stability

  • Store dehydrated medium in a dry environment at room temperature
  • Protect from moisture and direct light
  • Prepared medium should be used according to laboratory protocols

⚠️ Limitations & Precautions

  • Autoclaving will damage the medium and must be avoided
  • Do not use if powder shows caking, discoloration, or moisture absorption
  • Not intended for clinical diagnostic use

Product List: Clostridium, Lactic Acid Bacteria & Anaerobe Culture Media

1. Clostridium Media

  • Differential Reinforced Clostridial Agar (DRCA)
    • Spec: 250g/bottle
  • Differential Clostridial Agar (DCA)
    • Spec: 250g/bottle
  • Reinforced Clostridium Medium (RCM)
    • Spec: 250g/bottle
  • Reinforced Clostridium Agar
    • Spec: 250g/bottle
  • Sulfite Cycloserine Agar Base (SC Agar Base)
    • Spec: 250g/bottle

2. Lactic Acid Bacteria & Bifidobacterium Media

  • BBL Agar Medium
    • Spec: 250g/bottle
  • PYG Broth Medium Base
    • Spec: 250g/bottle
  • TPY Broth Medium
    • Spec: 250g/bottle
  • TPY Agar Medium
    • Spec: 250g/bottle
  • MRS Medium (Modified MRS Medium Base)
    • Spec: 250g/bottle
  • MC Medium (Modified MC Medium Base)
    • Spec: 250g/bottle
  • Bifidobacterium Biochemical Tests Base Medium
    • Spec: 100g/bottle
  • MRS Broth
    • Spec: 250g/bottle
  • Tomato Juice Agar, Modified
    • Spec: 250g/bottle
  • Bifidobacterium BS Medium
    • Spec: 250g/bottle
  • BLB Medium Base
    • Spec: 250g/bottle
  • Lactobacillus Selective Agar (LBS Agar)
    • Spec: 250g/bottle
  • Lactobacillus Selective Broth (LBS Broth)
    • Spec: 250g/bottle
  • M17 Agar
    • Spec: 1000ml/bottle (Note: Please confirm if this is liquid medium or if the unit should be grams)
  • M17 Broth
    • Spec: 1000ml/bottle
  • APT Agar
    • Spec: 250g/bottle
  • APT Broth
    • Spec: 250g/bottle
  • Raka-Ray 3# Medium
    • Spec: 250g/bottle

3. Anaerobe Culture Media

  • Wilkins-Chalgren Anaerobe Agar
    • Spec: 250g/bottle
  • Wilkins-Chalgren Anaerobe Broth
    • Spec: 250g/bottle
  • Anaerobe Broth MIC
    • Spec: 250g/bottle
  • CDC Anaerobe Agar Base
    • Spec: 250g/bottle
  • Anaerobic Agar
    • Spec: 250g/bottle
  • Brewer Anaerobic Agar
    • Spec: 250g/bottle
  • Thioglycollate Medium (Brewer)
    • Spec: 250g/bottle
  • Schaedler Agar
    • Spec: 250g/bottle
  • Schaedler Broth
    • Spec: 250g/bottle
  • CHO Medium Base
    • Spec: 250g/bottle
  • Anaerobic Broth Base
    • Spec: 250g/bottle
  • Anaerobic Agar Base
    • Spec: 250g/bottle

4. Gifu Anaerobic Medium (GAM) Series

  • Gifu Anaerobic Medium Broth (GAM Broth)
    • Spec: 250g/bottle
  • Gifu Anaerobic Medium Semisolid (GAM Semisolid)
    • Spec: 250g/bottle
  • Gifu Anaerobic Medium Semisolid without Dextrose
    • Spec: 250g/bottle
  • Gifu Anaerobic Medium Agar (GAM Agar)
    • Spec: 250g/bottle
  • Gifu Anaerobic Medium Broth Base, Modified
    • Spec: 250g/bottle
  • Gifu Anaerobic Medium Agar Base, Modified
    • Spec: 250g/bottle

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