Dubos Oleic Agar Base – 250g for Mycobacterium tuberculosis Cultivation

Dubos Oleic Agar Base is a selective medium designed for the isolation and cultivation of Mycobacterium tuberculosis. When used with Dubos Oleic Agar Base Additive and penicillin, this medium supports optimal growth of tuberculosis-causing bacteria. The formula contains casein digest, asparagine, essential salts, and agar to maintain nutrient balance and stable pH.

Product Information

• Product Name: Dubos Oleic Agar Base

• Pack Size: 250g per bottle

• Form: Dehydrated powder

• Storage: Room temperature

• Shelf Life: 3 years

Applications

• Isolation and cultivation of Mycobacterium tuberculosis

• Laboratory and clinical microbiology research

• Requires addition of Dubos Oleic Agar Base Additive and penicillin for selective cultivation

Composition (per liter)

• Pancreatic Digest of Casein – 0.5g

• Asparagine – 1.0g

• Monopotassium Phosphate – 1.0g

• Disodium Phosphate (anhydrous) – 2.5g

• Ferric Ammonium Citrate – 0.05g

• Magnesium Sulfate – 0.01g

• Calcium Chloride – 0.5mg

• Zinc Sulfate – 0.1mg

• Copper Sulfate – 0.1mg

• Agar – 15.0g

• pH 6.6 ± 0.2 (25℃)

Preparation Instructions

1. Dissolve 2.0g of Dubos Oleic Agar Base in 90ml distilled or deionized water and heat until completely dissolved.

2. Sterilize at 121℃ for 15 minutes.

3. After sterilization, shake gently to prevent agar sedimentation and cool to approximately 55℃.

4. Aseptically add 10ml Dubos Oleic Agar Base Additive (2 vials) and 2,500–5,000 U penicillin (0.5–1 vial penicillin solution).

5. Mix gently and pour plates for cultivation.

Notes

• The powder may settle or clump, but this does not affect medium performance.

• Ensure aseptic technique when adding additives and penicillin.