Dehydrated Dnase Test Agar with Toluidine Blue powder, 100g container for nuclease detection culture medium preparation

DNase Test Agar with Toluidine Blue | Dehydrated Culture Medium for DNase Detection 100g

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DNase Test Agar with Toluidine Blue

DNase Test Agar with Toluidine Blue is a microbiological culture medium used for detecting DNase enzyme activity in bacteria and other microorganisms.

The medium contains deoxyribonucleic acid (DNA) and toluidine blue dye, which allows visual detection of DNA hydrolysis. When DNase-producing microorganisms grow on the medium, degradation of DNA results in distinct color changes around microbial colonies, enabling clear identification.

This medium is commonly used in microbial differentiation, microbiological diagnostics, and laboratory research.

The dehydrated powder formulation provides stable storage, easy preparation, and consistent performance for microbiological testing.

Key Features

  • Designed for DNase enzyme activity detection
  • Contains toluidine blue indicator
  • Clear visualization of DNA hydrolysis reactions
  • Reliable microbiological culture medium
  • Dehydrated powder format for convenient storage
  • Suitable for microbiology laboratories and research applications

Medium Composition (per liter)

Deoxyribonucleic Acid ……………… 2.0 g
Pancreatic Digest of Casein ………. 10.0 g
Papaic Digest of Soybean Meal ……. 10.0 g
Sodium Chloride …………………….. 5.0 g
Toluidine Blue ………………………. 0.1 g
Agar …………………………………. 15.0 g

pH: 7.3 ± 0.2 (25°C)


Preparation Instructions

  1. Suspend 42.0 g of the medium in 1 liter of distilled or deionized water.
  2. Heat and boil until the medium is completely dissolved.
  3. Dispense into suitable containers.
  4. Sterilize in an autoclave at 121°C for 15 minutes.
  5. After sterilization, mix gently to prevent agar from settling at the bottom before solidification.

Storage

Store tightly closed in a cool and dry place at room temperature.
Shelf life: 3 years.


Product Specifications

ParameterSpecification
Product NameDNase Test Agar with Toluidine Blue
FormatDehydrated Powder
Net Content100 g
StorageRoom Temperature
Shelf Life3 Years
ApplicationDNase Detection

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