
Dnase Test Agar with Methyl Green Powder 100g
Dnase Test Agar with Methyl Green is a dehydrated culture medium used in nuclease testing.
It is supplied as a dry powder in 100g bottles, with a shelf life of 3 years when stored refrigerated.
Composition per liter:
Deoxyribonucleic Acid (DNA): 2.0 g
Pancreatic Digest of Casein: 10.0 g
Proteose Peptone No. 3: 10.0 g
Sodium Chloride: 5.0 g
Methyl Green: 0.05 g
Agar: 15.0 g
Final pH: 7.3 ± 0.2 (at 25°C)
Preparation:
Suspend 42.0 g of the powder in 1 L of distilled or deionized water. Heat to boiling until fully dissolved, dispense into suitable containers, and sterilize by autoclaving at 121°C for 15 minutes. Mix well after sterilization to prevent agar sedimentation.