DNase Test Agar with Methyl Green
DNase Test Agar with Methyl Green is a microbiological culture medium formulated for the detection of DNase enzyme activity produced by microorganisms.
The medium contains deoxyribonucleic acid (DNA) combined with methyl green dye, allowing visual detection of DNA hydrolysis. Microorganisms producing DNase degrade DNA, resulting in distinct zones around colonies.
This medium is commonly used in microbial differentiation, laboratory diagnostics, and microbiological research.
The dehydrated powder formulation provides stable storage, convenient preparation, and consistent laboratory performance.
Key Features
- Designed for DNase enzyme activity detection
- Contains methyl green indicator
- Clear visualization of DNA hydrolysis zones
- Reliable microbiological culture medium
- Dehydrated powder for easy storage and preparation
- Suitable for microbiology laboratories and research
Medium Composition (per liter)
Deoxyribonucleic Acid ……………… 2.0 g
Pancreatic Digest of Casein ………. 10.0 g
Proteose Peptone No. 3 …………… 10.0 g
Sodium Chloride …………………….. 5.0 g
Methyl Green ………………………… 0.05 g
Agar …………………………………. 15.0 g
pH: 7.3 ± 0.2 (25°C)
Preparation Instructions
- Suspend 42.0 g of the medium in 1 liter of distilled or deionized water.
- Heat and boil until the medium is completely dissolved.
- Dispense into suitable containers.
- Sterilize in an autoclave at 121°C for 15 minutes.
- After sterilization, gently mix the medium to prevent agar from settling at the bottom before solidification.
Storage
Store tightly closed in a refrigerated environment.
Shelf life: 3 years.
Product Specifications
| Parameter | Specification |
|---|---|
| Product Name | DNase Test Agar with Methyl Green |
| Format | Dehydrated Powder |
| Net Content | 100 g |
| Storage | Refrigerated |
| Shelf Life | 3 Years |
| Application | DNase Detection |