DNase Test Agar – 100g | Deoxyribonuclease Detection
Specialized Medium for DNase Enzyme Detection
DNase Test Agar contains 2g/L DNA substrate to detect deoxyribonuclease activity in microorganisms. The clear differentiation between positive and negative reactions makes it ideal for bacterial identification and characterization.
Key Applications:
– Staphylococcus aureus identification
– Bacterial taxonomy and classification
– Clinical isolate characterization
– Microbiology education and research
– Quality control in diagnostic laboratories
Technical Specifications:
– Formula (per liter):
– Deoxyribonucleic Acid: 2.0g
– Pancreatic Digest of Casein: 15.0g
– Papaic Digest of Soybean Meal: 5.0g
– Sodium Chloride: 5.0g
– Agar: 15.0g
– pH: 7.3 ± 0.2 (25°C)
– Packaging: 100g/bottle
– Storage: Room temperature (15-25°C)
– Shelf Life: 3 years
Test Protocol:
1. Suspend 42.0g in 1L distilled water
2. Boil with stirring until completely dissolved
3. Autoclave at 121°C for 15 minutes
4. Inoculate and incubate at 35°C for 18-24 hours
5. Flood with 1M HCl or 0.1% toluidine blue solution
Result Interpretation:
– With HCl: Clear zones around DNase-positive colonies
– With toluidine blue: Blue (negative) vs pink (positive)