D/E Neutralizing Broth Base – 250g | Cosmetic Preservative Neutralization

Complete Preservative Neutralization for Cosmetic Microbiology

D/E Neutralizing Broth Base effectively inactivates common cosmetic preservatives and disinfectants, enabling accurate microbial recovery from personal care products. Its lecithin-polysorbate system meets global standards for preservative efficacy testing.

Key Applications:

– Preservative efficacy testing (PET)

– Cosmetic product microbial limits testing

– Raw material bioburden assessment

– Sanitizer validation studies

– Topical product contamination screening

Technical Specifications:

– Formula (per liter):

– Pancreatic Digest of Casein: 5.0g

– Glucose: 10.0g

– Soybean Lecithin: 7.0g

– Sodium Thiosulfate: 6.0g

– Sodium Hydrogensulfite: 2.5g

– Yeast Extract: 2.5g

– Sodium Thioglycollate: 1.0g

– Bromcresol Purple: 0.02g

– pH: 7.6 ± 0.2 (25°C)

– Packaging: 250g/bottle

– Storage: Room temperature (15-25°C)

– Shelf Life: 3 years

Preparation Protocol:

1. Suspend 34.0g in 1L distilled water

2. Heat to 80°C with vigorous stirring

3. Add 1g polysorbate 80 per 200ml broth

4. Autoclave at 121°C for 15 minutes

Note: Initial clumping is normal – continue heating to dissolve

Quality Notes:

– Validated neutralization capacity

– Low background bioburden

– Consistent batch-to-batch performance