D/E Neutralizing Agar Base – 250g | Cosmetic Preservative Inactivation
Complete Preservative Neutralization for Solid Media
D/E Neutralizing Agar Base provides reliable inactivation of cosmetic preservatives in solid culture systems. The optimized lecithin-polysorbate formula ensures accurate microbial recovery from personal care products on plates.
Key Applications:
– Preservative efficacy testing (PET)
– Cosmetic microbial limits testing
– Cream/lotion contamination screening
– GMP facility environmental monitoring
– Raw material quality control
Technical Specifications:
– Formula (per liter):
– Pancreatic Digest of Casein: 5.0g
– Glucose: 10.0g
– Soybean Lecithin: 7.0g
– Sodium Thiosulfate: 6.0g
– Sodium Hydrogensulfite: 2.5g
– Yeast Extract: 2.5g
– Sodium Thioglycollate: 1.0g
– Bromcresol Purple: 0.02g
– Agar: 15.0g
– pH: 7.6 ± 0.2 (25°C)
– Packaging: 250g/bottle
– Storage: Room temperature (15-25°C)
– Shelf Life: 3 years
Preparation Protocol:
1. Suspend 49.0g in 1L distilled water
2. Boil ≥1 minute with vigorous stirring
3. Add 1g polysorbate 80 per 200ml medium
4. Autoclave at 121°C for 15 minutes
5. Swirl during cooling to ensure even mixing
Quality Notes:
– Validated neutralization capacity
– pH indicator for visual monitoring
– Batch-to-batch consistency
