Columbia Broth – 250g | Fastidious Microorganism Culture

Premium Enrichment Broth for Demanding Microorganisms

Columbia Broth provides optimal growth conditions with its unique combination of beef heart digest (3.0g/L), triple nitrogen sources, and Tris buffer system for pH stability.

Key Applications:

– Clinical pathogen isolation

– Anaerobic bacteria cultivation

– Vaccine production seed cultures

– Industrial strain maintenance

– Research on nutritionally sensitive organisms

Technical Specifications:

– Formula (per liter):

– Yeast Extract: 5.0g

– Proteose Peptone: 5.0g

– Pancreatic Digest of Casein: 10.0g

– Beef Heart Digest: 3.0g

– L-Cysteine HCl: 0.1g

– Dextrose: 2.5g

– Sodium Chloride: 5.0g

– Tris Buffer System: 3.69g

– pH: 7.5 ± 0.2 (25°C)

– Packaging: 250g/bottle

– Storage: Room temperature (15-25°C)

– Shelf Life: 3 years

Preparation Protocol:

1. Suspend 35.0g in 1L distilled water

2. Boil with continuous stirring until dissolved

3. Autoclave at 121°C for 15 minutes

Quality Advantages:

– Tris-buffered for pH stability

– Reduced components (L-cysteine) for anaerobes

– Balanced micronutrients (Fe/Mg)