Columbia Blood Agar Base 250g – Culture Medium for Blood Agar Preparation

Overview
Columbia Blood Agar Base is a high-quality culture medium widely used for the cultivation of bacteria, particularly fastidious microorganisms. When supplemented with sterile defibrinated sheep blood, it provides a nutrient-rich environment suitable for isolation and growth of demanding bacterial species. It is commonly applied in clinical, research, and diagnostic microbiology laboratories.

Composition (per liter)

• Pancreatic Digest of Casein: 10.0 g

• Yeast Extract: 5.0 g

• Peptic Digest of Meat: 5.0 g

• Beef Heart Digest: 3.0 g

• Sodium Chloride: 5.0 g

• Corn Starch: 1.0 g

• Agar: 15.0 g

• pH: 7.3 ± 0.2 (at 25°C)

Preparation Instructions

1. Weigh 44.0 g of Columbia Blood Agar Base powder and add to 1 L of distilled or deionized water.

2. Heat with stirring and boil for at least 1 minute until fully dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121°C for 15 minutes or at 115°C for 30 minutes.

5. Cool to 45–50°C.

6. Under aseptic conditions, add 5–7% sterile defibrinated sheep blood (pre-warmed to 35°C).

7. Mix gently and pour into plates for use.

Key Features

• Suitable for cultivation of a wide range of bacteria, especially fastidious organisms

• Optimized for blood agar preparation with added sheep blood

• Reliable performance for clinical and research microbiology

• Easy to prepare with consistent quality

Applications

• Preparation of blood agar plates

• Cultivation of fastidious microorganisms

• Clinical diagnostics and pathogen isolation

• Microbiological research and teaching laboratories