Product Overview
CN Agar Base is a microbiological culture medium formulated for the selective detection and enumeration of Pseudomonas aeruginosa using membrane filtration techniques.
The dehydrated powder medium is dissolved in purified water, sterilized, and supplemented with glycerol and nalidixic acid solution prior to plate preparation. This culture medium is commonly used in microbiology laboratories performing environmental monitoring, water quality testing, and research involving Pseudomonas species.
The formulation contains selective agents that support the detection of target organisms in microbiological testing procedures.
Product Specifications
| Parameter | Description |
|---|---|
| Product Name | CN Agar Base |
| Application | Detection of Pseudomonas aeruginosa |
| Method | Membrane filtration method |
| Package | 250 g / bottle |
| Storage | Room temperature |
| Shelf Life | 3 years |
| Form | Dehydrated powder |
Applications
CN Agar Base is commonly used for:
- Detection of Pseudomonas aeruginosa
- Membrane filtration microbiological testing
- Water microbiology analysis
- Environmental monitoring laboratories
- Industrial microbiology quality control
- Research laboratories
Medium Composition (per liter)
Pancreatic Digest of Gelatin — 16.0 g
Tryptone — 10.0 g
Potassium Sulfate — 10.0 g
Magnesium Chloride — 1.4 g
Cetrimide — 0.2 g
Agar — 15.0 g
Final pH: 7.1 ± 0.2 (25°C)
Preparation Instructions
- Suspend 5.26 g of the medium in 100 ml distilled or deionized water.
- Add 1 ml glycerol.
- Sterilize at 121°C for 15 minutes or 115°C for 30 minutes.
- After sterilization, mix well to prevent agar from settling at the bottom of the container.
- Cool the medium to approximately 50°C.
- Aseptically add 1 vial nalidixic acid solution (1.5 mg).
- Mix thoroughly and pour into sterile petri plates.
- Allow the plates to solidify before use.
Note:
Due to the presence of cetrimide, the prepared medium may appear slightly turbid.