Chapman Stone Agar Medium for Staphylococcus aureus Isolation | Dehydrated Culture Medium 250g

Chapman Stone Agar Medium

Chapman Stone Agar Medium is a microbiological culture medium formulated for the isolation and cultivation of Staphylococcus aureus.

The medium contains high salt concentration and mannitol, which create selective conditions suitable for the growth of Staphylococcus species while inhibiting many other microorganisms.

This dehydrated powder medium provides consistent composition, convenient storage, and reliable performance for microbiology laboratories performing bacterial isolation and research.

It is suitable for clinical microbiology, food safety analysis, environmental testing, and laboratory microbial studies.

Key Features

• Designed for Staphylococcus aureus isolation
• Selective high-salt microbiological culture medium
• Dehydrated powder format for easy preparation
• Suitable for microbiology laboratories and research
• Reliable microbiological culture medium formulation
• Consistent performance in bacterial isolation procedures

Medium Composition (per liter)

Ammonium Sulfate ((NH₄)₂SO₄) ………. 75.0 g
D-Mannitol ……………………………… 10.0 g
Sodium Chloride ………………………… 55.0 g
Dipotassium Phosphate (K₂HPO₄) ……. 5.0 g
Gelatin ………………………………….. 30.0 g
Yeast Extract ……………………………. 2.5 g
Tryptone ………………………………… 10.0 g
Agar ……………………………………… 15.0 g

Preparation Instructions

1. Suspend 202.5 g of the medium in 1 liter of distilled or deionized water.
2. Heat and boil for approximately 1 minute until the medium is completely dissolved.
3. Adjust the volume to 1000 ml if necessary.
4. Dispense into suitable containers.
5. Sterilize at 121°C for 15 minutes or 116°C for 30 minutes.
6. After sterilization, mix well to prevent agar from settling at the bottom before solidification.
7. Cool to approximately 55°C before use.

Storage

Store tightly sealed in a cool and dry place at room temperature.

Shelf life: 3 years

Product Specifications