CFAT Agar Base
CFAT Agar Base (Cadmium Sulfate Fluoride Acridine Trypticase Agar Base) is a selective microbiological agar medium designed for the isolation and cultivation of Actinomyces species, including Actinomyces naeslundii and Actinomyces viscosus.
This selective agar culture medium is supplied as a dehydrated powder, allowing convenient preparation in microbiology laboratories. When supplemented with appropriate additives, the medium provides a selective environment suitable for isolating actinomycetes from mixed microbial populations.
The formulation is commonly used in microbiology laboratories, clinical microbiology research, and studies involving oral microbiota and actinomycete isolation.
For optimal selectivity, the medium requires supplementation with acriflavine solution, basic fuchsin solution, and optionally sterile defibrinated sheep blood before use.
Key Features
- Selective medium for Actinomyces isolation
- Suitable for Actinomyces naeslundii and Actinomyces viscosus
- Selective microbiological agar culture medium
- Supplied as dehydrated powder
- Compatible with blood supplementation
- Suitable for microbiology and clinical research laboratories
- 250 g laboratory packaging
- Stable formulation with 3-year shelf life
Applications
- Isolation of Actinomyces species
- Oral microbiology research
- Clinical microbiology studies
- Selective cultivation of actinomycetes
- Laboratory microbial culture studies
Medium Composition
Composition (per liter)
Cadmium Sulfate
Sodium Fluoride
Acridine
Trypticase
Agar
(Additional supplements are required during preparation as described in the instructions.)
Required Supplements
The following supplements are required during preparation:
Acriflavin Solution — 0.12 mg
Basic Fuchsin Solution
Sterile Defibrinated Sheep Blood (optional, typically 5%)
Directions for Use
- Suspend 50.0 g of the medium in 980 ml distilled or deionized water.
- Add 10 vials of acriflavin solution and 10 vials of basic fuchsin solution.
- Heat and boil for at least 1 minute until completely dissolved.
- Dispense into suitable containers.
- Sterilize by autoclaving at 121°C for 15 minutes.
- Mix thoroughly after sterilization.
- Pour into sterile petri dishes and allow the medium to solidify.
- If required, add 5% sterile defibrinated sheep blood for selective cultivation.
Product Specifications
| Item | Description |
|---|---|
| Product Name | CFAT Agar Base |
| Full Name | Cadmium Sulfate Fluoride Acridine Trypticase Agar Base |
| Medium Type | Selective Agar Medium |
| Format | Powder |
| Package | 250 g / bottle |
| Storage | Room temperature |
| Shelf Life | 3 years |
| Application | Actinomyces isolation |