Casein Soybean Digest Broth – 250g | Microbial Culture Media for Sterility Testing
Casein Soybean Digest Broth is a general-purpose culture medium designed for reliable bacterial growth in pharmaceutical quality control, environmental monitoring, and diagnostic applications.
Key Applications:
✔️ Sterility testing of medical devices & parenteral products
✔️ Bioburden testing according to USP <61> and EP 2.6.12
✔️ Enrichment culture for fastidious microorganisms
✔️ Growth promotion testing of culture media
Optimized Formula Per Liter:
| Component | Quantity |
|————————————|———-|
| Pancreatic Digest of Casein | 17.0 g |
| Enzymatic Digest of Soybean Meal | 3.0 g |
| Sodium Chloride | 5.0 g |
| Dextrose | 2.5 g |
| Dipotassium Hydrogen Phosphate | 2.5 g |
Final pH: 7.3 ± 0.2 (25°C)
Simplified Protocol:
1️⃣ Dissolve 30.0g powder in 1L distilled water
2️⃣ Sterilize by autoclaving at 121°C for 15 minutes
3️⃣ Cool to 45-50°C before use
Product Advantages:
– Dual enzymatic digests: Pancreatic casein + Papain soybean for balanced nutrient profile
– Buffered system: K₂HPO₄ maintains pH stability during growth
– Ready-to-use powder: Reduces preparation errors
– 3-year shelf life: Ambient storage without viability loss
Quality Assurance:
– Batch-to-batch consistency
– Low particulate content for clear broth observation
