CAMHB Broth – Calcium-Enriched, Magnesium-Free Aerobic Enrichment Medium (250g)
Specialized CAMHB Broth for Clinical Aerobic Microorganism Enrichment
This cation-adjusted broth contains 0.05g/L calcium chloride while excluding magnesium ions, creating optimal conditions for rapid aerobic microorganism recovery from clinical specimens. The unique 17.5g/L casamino acids formulation meets CLSI standards for antimicrobial susceptibility testing preparations.
Critical Applications:
🏥 Clinical Microbiology: Primary enrichment for blood cultures and sterile fluids
💊 AST Preparation: CLSI-compliant base for broth microdilution tests
🧫 Fastidious Organisms: Supports growth of Streptococci and HACEK group
Technical Advantages:
✅ Cation Optimization: Ca²⁺ (0.05g/L) without Mg²⁺ interference
✅ Nitrogen-Rich: 17.5g/L casamino acids for rapid biomass accumulation
✅ Starch Protection: 1.5g/L soluble starch prevents autolysis
✅ Validated Sterilization: 116-121°C for 10 minutes
Preparation Protocol:
Suspend 22.0g in 1L purified water
Warm to 45-50°C for complete dissolution
Autoclave at 116-121°C for 10 minutes
Complete Formulation (Per Liter):
Beef Extract: 3.0g
Casamino Acids: 17.5g
Soluble Starch: 1.5g
CaCl₂: 0.05g
