C.L.E.D Medium for Isolation and Cultivation of Urinary Pathogens – 250g

C.L.E.D Medium (Cystine Lactose Electrolyte Deficient) is a specialized microbiological medium designed for the isolation and cultivation of urinary tract pathogens. It is also suitable for deep culture storage and transport. The medium promotes the growth of a wide range of uropathogens while allowing differentiation based on lactose fermentation and pH indicators.

Key Features:

• Ideal for urinary pathogen isolation and cultivation.

• Supports deep culture storage and transport of clinical samples.

• High-quality ingredients ensure reliable and reproducible results.

• Sterilization ready: Easily prepared by autoclaving.

• Long shelf life: Stable for up to 3 years at room temperature.

Instructions for Use:
Dissolve 34.2g of C.L.E.D Medium in 1L of distilled or deionized water. Boil until completely dissolved. Sterilize by autoclaving at 121°C for 15 minutes. After sterilization, gently shake to mix and prevent agar from settling. Cool and store until use.

Storage: Store at room temperature, away from direct sunlight. Shelf life: 3 years.

Ingredients per liter:

• Pancreatic Digest of Casein: 4.0g

• Peptone: 4.0g

• Beef Extract: 3.0g

• Lactose: 10.0g

• L-Cystine: 0.128g

• Bromthymol Blue: 0.02g

• Agar: 13.0g

• pH: 7.3 ± 0.2 at 25°C