Burkholderia cepacia Selective Agar Base (BCSA) – 250g
Burkholderia cepacia Selective Agar Base (BCSA) is a specialized medium designed for the selective isolation and cultivation of Burkholderia cepacia. This 250g bottle provides a high-quality base that, when combined with BCSA supplement, creates an optimal environment to support the growth of target bacteria while inhibiting other microorganisms, making it ideal for microbiological testing applications.
Key Features
Primary Use: Specifically formulated for the selective isolation and cultivation of Burkholderia cepacia. Requires adding 1 vial of BCSA supplement per 200ml of medium during preparation.
Formula (per liter): Contains Casein Peptone (10.0g), Lactose (10.0g), Sucrose (10.0g), Sodium Chloride (5.0g), Yeast Extract (1.5g), Phenol Red (0.08g), Crystal Violet (0.002g), and Agar (14.0g), with a pH of 6.8±0.3 at 25°C.
Storage: Stable at room temperature with a 3-year shelf life, ensuring consistent performance over time.
Preparation Instructions
Measure 50.58g of the base and dissolve in 1L of distilled or deionized water.
Heat to a boil for at least 1 minute to ensure complete dissolution, then dispense into containers.
Sterilize at 121°C under high pressure for 15 minutes. Shake immediately after sterilization.
Cool to 50-55°C, then aseptically add 1 vial of BCSA supplement per 200ml of medium. Mix gently and pour into petri dishes for use.
This BCSA base is a reliable tool for laboratories requiring accurate selective isolation of Burkholderia cepacia.
