Buffered Listeria Enrichment Broth Base (BLEB) – Selective Enrichment Medium, 250g

Buffered Listeria Enrichment Broth Base (BLEB) provides a reliable medium for the selective enrichment of Listeria in microbiology laboratories.

Composition (per liter):

• Pancreatic Digest of Casein – 17.0g

• Soytone – 3.0g

• Sodium chloride – 5.0g

• Dextrose – 2.5g

• Dipotassium phosphate – 2.5g

• Yeast extract – 6.0g

• Disodium hydrogen phosphate – 9.6g

• Potassium dihydrogen phosphate – 1.35g

• Sodium pyruvate – 1.1g

• pH 7.3 ± 0.1 (25℃)

Preparation Instructions:

1. Dissolve 48.0g of the medium in 1L distilled or deionized water with gentle warming and stirring.

2. Dispense 225ml portions into bottles.

3. Sterilize at 121℃ for 15 minutes.

4. Cool to room temperature, add 25.0g sample, and incubate at 30℃ for 4 hours.

5. Supplement each 225ml culture with Acriflavin solution (2.25mg), Listeria selective supplement, and Nalidixic acid solution, mix well, and continue incubation at 30℃ for up to 48 hours.

Storage: Store at room temperature. Shelf life up to 3 years.