Broth Medium for Enumeration of Phosphobacteria | Phosphate Bacteria Counting Medium

Broth Medium for Enumeration of Phosphobacteria is a solid medium designed for counting phosphate-solubilizing bacteria in soil and fertilizer samples. It provides a nutrient-rich environment for accurate enumeration of bacterial colonies.

Applications:

• Determination of phosphate-solubilizing bacterial counts

• Soil and fertilizer microbiology studies

• Agricultural and environmental microbiology research

Specifications:

• Format: Powder, 250 g/bottle

• Storage: Room temperature

• Shelf Life: 3 years

Formula (per liter):

• Peptone – 5.0 g

• Beef Extract – 5.0 g

• Sodium Chloride – 5.0 g

• Agar – 20.0 g

• Final pH: 6.9 ± 0.1 (25℃)

Preparation Instructions:

1. Dissolve 35.0 g of the medium in 1 liter of distilled or deionized water.

2. Heat to boiling until completely dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121℃ for 15 minutes.

5. Mix well after sterilization.

6. Pour plates and allow to solidify before use.