Bromcresol Purple Peptone Broth – Sterilization Testing Medium

Colorimetric Sterility Testing Medium with Dual Carbohydrates

Bromcresol Purple Peptone Broth provides visual pH indication (purple→yellow) for microbial growth detection in sterilization validation. Contains 5g/L glucose and 1g/L soluble starch for enhanced sensitivity.

Key Applications:

– Medical device sterility testing

– Disinfectant efficacy validation

– Pharmaceutical sterilization cycle monitoring

– Food processing equipment sanitation checks

– Industrial bioburden assessment

Technical Specifications:

– Formula (per liter):

– Peptone: 10.0g

– Dextrose: 5.0g

– Soluble Starch: 1.0g

– Bromcresol Purple: 0.02g

– pH: 7.1 ± 0.1 (25°C)

– Preparation:

1. Suspend 16.0g in 1L distilled water

2. Dissolve at 45-50°C

3. Sterilize at 115°C for 30 minutes

Visual Indicators:

– Purple: Sterile (pH >7.0)

– Yellow: Contaminated (pH <6.8)

Quality Features:

– Starch-glucose synergy for broad detection

– Low particulate formulation

– Batch-to-batch consistency