Brilliant Green Phenol Red Agar – Selective Agar Medium for Salmonella Isolation, 250g

Brilliant Green Phenol Red Agar (BGPA) is a selective and differential culture medium formulated for the isolation of Salmonella species from clinical, food, and environmental specimens. The medium contains brilliant green dye, which selectively inhibits Gram-positive bacteria and many Gram-negative organisms, while phenol red serves as a pH indicator to detect carbohydrate fermentation.

Product Information

• Product Name: Brilliant Green Phenol Red Agar (BGPA)

• Pack Size: 250g per bottle

• Form: Dehydrated culture medium powder

• Storage: Store at room temperature

• Shelf Life: 3 years

Medium Composition (per liter)

• Peptone – 10.0 g

• Beef Extract – 5.0 g

• Yeast Extract – 3.0 g

• Disodium Hydrogen Phosphate – 1.0 g

• Monosodium Phosphate – 0.6 g

• Lactose – 10.0 g

• Sucrose – 10.0 g

• Phenol Red – 0.09 g

• Brilliant Green – 0.0125 g

• Agar – 15.0 g

• Final pH: 7.0 ± 0.2 (25°C)

Applications

• Selective isolation of Salmonella spp.

• Food microbiology and contamination testing

• Clinical diagnostic laboratories

• Environmental monitoring

Preparation Instructions

1. Suspend 54.7 g of the medium powder in 1 liter of distilled or deionized water.

2. Heat with frequent agitation until completely dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121°C for 15 minutes (or at 115°C for 30 minutes).

5. Mix thoroughly after sterilization to avoid agar sedimentation.

6. Allow to solidify before use.

Safety Notes

• Laboratory use only.

• Handle Salmonella cultures using appropriate biosafety procedures.