Brilliant Green Lactose Bile Broth (BGLB) – Medium for E. coli and Coliform Testing, 250g

Brilliant Green Lactose Bile Broth (BGLB) is a selective medium used for detection and enumeration of Escherichia coli and coliform bacteria. Supplied in 250g bottles, it is stable at room temperature with a shelf life of 3 years, suitable for microbiology laboratories performing water, food, and environmental testing.

Composition (per liter):

• Peptone: 10.0g

• Lactose: 10.0g

• Oxgal (Bovine Bile Powder): 20.0g

• Brilliant Green: 0.0133g

pH: 7.2 ± 0.1 (25°C)

Usage Instructions:
Dissolve 40.0g of medium in 1L of distilled or deionized water (adjust proportionally if preparing more or less). Warm gently to dissolve completely, dispense, and sterilize at 121°C for 15 minutes. Cool and use for testing E. coli and coliform bacteria.