Brain Heart Infusion Agar (BHIA) 250g – Culture Medium for Fastidious Bacteria

Overview
Brain Heart Infusion Agar (BHIA) is a highly nutritious solid culture medium formulated for the growth of a broad range of microorganisms, especially fastidious bacteria. It combines animal tissue digests with brain and heart infusions to provide essential growth factors, while agar solidifies the medium for plate cultivation. This medium is widely used in clinical, industrial, and research microbiology for isolating and maintaining bacterial cultures.

Composition (per liter)

• Enzymatic Digest of Animal Tissues: 10.0 g

• Sodium Chloride: 5.0 g

• Dextrose: 2.0 g

• Disodium Phosphate: 2.5 g

• Dehydrated Calf Brain Infusion: 12.5 g

• Dehydrated Beef Heart Infusion: 5.0 g

• Agar: 15.0 g

• pH: 7.4 ± 0.2 (at 25°C)

Preparation Instructions

1. Weigh 52.0 g of BHIA powder and add to 1 L of distilled or deionized water.

2. Heat to boiling until completely dissolved.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121°C for 15 minutes.

5. Mix gently after sterilization and pour into plates under aseptic conditions.

Key Features

• Rich nutrient formulation supports both fastidious and non-fastidious bacteria

• Solid medium suitable for colony isolation and culture maintenance

• Reliable performance in clinical, industrial, and research microbiology

Applications

• Cultivation of fastidious microorganisms

• Isolation and enumeration of bacteria from clinical and environmental samples

• Use in quality control, food safety testing, and research laboratories