Blood Agar Base No.2 – Premium Medium for 5% Sheep Blood Agar Plates (250g)
Clinical-Grade Blood Agar Base No.2 for Pathogen Isolation & Hemolysis Studies
Optimized Blood Agar Base No.2 provides the ideal foundation for preparing 5% defibrinated sheep blood agar plates, essential for clinical bacteriology. With high-peptone formulation (23.0g/L) and pH-stabilized at 7.4±0.2, this medium ensures superior hemolysis visualization and fastidious organism recovery when supplemented with blood.
Key Applications:
🩸 Clinical Diagnostics: Isolation of Streptococcus, Staphylococcus and other fastidious pathogens
🔍 Hemolysis Studies: Clear differentiation of alpha/beta/gamma hemolytic reactions
🧫 Routine Culture: Standard medium for clinical microbiology laboratories
Technical Highlights:
✅ Enriched Formula: 23.0g/L special peptone for enhanced growth
✅ Precision pH: 7.4±0.2 at 25°C for optimal hemolysis patterns
✅ Blood-Ready: Designed for 5% defibrinated sheep blood addition
✅ Easy Preparation: 40.0g/L standardized concentration
Blood Agar Preparation Protocol:
Dissolve 40.0g in 1L purified water
Autoclave at 121°C for 15 minutes
Critical Step: Shake while cooling to prevent agar sedimentation
At 45-50°C, aseptically add pre-warmed (35°C) 5% sheep blood
Mix gently and pour plates
Complete Formulation (Per Liter):
Special Peptone: 23.0g
Sodium Chloride: 5.0g
Agar: 12.0g
