Blood Agar Base 250g – Culture Medium for Blood Agar Preparation

Overview
Blood Agar Base is a high-quality culture medium used for the preparation of blood agar plates. When supplemented with sterile defibrinated sheep blood, it supports the growth of a wide variety of microorganisms, including fastidious bacteria. This medium is widely applied in clinical, research, and diagnostic microbiology laboratories for bacterial isolation, cultivation, and hemolysis observation.

Composition (per liter)

• Peptone: 10.0 g

• Beef Extract: 3.0 g

• Sodium Chloride: 5.0 g

• Agar: 15.0 g

• pH: 7.3 ± 0.1 (at 25°C)

Preparation Instructions

1. Weigh 33.0 g of Blood Agar Base powder and add to 1 L of distilled or deionized water (adjust quantities proportionally if preparing a different volume).

2. Heat to boiling until fully dissolved.

3. Dispense into appropriate containers.

4. Sterilize by autoclaving at 121°C for 15 minutes or at 115°C for 30 minutes.

5. Shake gently after sterilization to prevent agar settling.

6. Cool to 50–55°C.

7. Under aseptic conditions, slowly add 5–7% sterile defibrinated sheep blood (pre-warmed to 35°C) along the inner wall of the container.

8. Mix gently and pour into plates; allow to solidify before use.

Key Features

• Designed for preparation of sheep blood agar plates

• Suitable for growth of a wide range of bacteria, including fastidious organisms

• Consistent quality for reliable laboratory results

• Easy preparation with clear instructions

Applications

• Blood agar plate preparation

• Cultivation of fastidious and non-fastidious bacteria

• Clinical diagnostic testing

• Microbiological research and teaching labs