Bi-Anti Chocolate Agar Base – 250g for Cultivation of Haemophilus and Neisseria

Bi-Anti Chocolate Agar Base is a specialized culture medium designed for the cultivation of Haemophilus species and Neisseria species. When supplemented with defibrinated sheep blood and selective antimicrobial agents, the medium provides enriched nutrients and optimal conditions for fastidious organisms while inhibiting contaminating flora.

Product Information

• Product Name: Bi-Anti Chocolate Agar Base

• Pack Size: 250g per bottle

• Form: Dehydrated culture medium powder

• Storage: Store at room temperature

• Shelf Life: 3 years

Applications

• Cultivation of Haemophilus influenzae

• Isolation of Neisseria gonorrhoeae and Neisseria meningitidis

• Clinical and diagnostic microbiology laboratories

• Research on pathogenic fastidious organisms

Key Features

• Nutrient-rich formulation for growth of fastidious bacteria

• Supports selective isolation with antibiotic supplementation

• Produces characteristic chocolate-brown medium after heating with blood

• Reliable performance in clinical diagnostics

Medium Composition (per liter)

• Pancreatic Digest of Casein: 10.0 g

• Yeast Extract: 5.0 g

• Peptic Digest of Meat: 5.0 g

• Beef Heart Digest: 3.0 g

• Sodium Chloride: 5.0 g

• Corn Starch: 1.0 g

• Agar: 15.0 g

• Final pH: 7.3 ± 0.2 (25℃)

Preparation Instructions

1. Suspend 44.0 g of medium in 1 L distilled or deionized water.

2. Heat to boiling until fully dissolved.

3. Dispense into suitable containers and autoclave at 121℃ for 15 minutes.

4. Shake well after sterilization to prevent agar settling.

5. Cool to 80–90℃, aseptically add 5–10% defibrinated sheep blood.

6. Maintain at ~80℃ with gentle shaking for ~10 minutes until medium turns chocolate-brown.

7. Cool to 45–50℃, aseptically add one vial of selective supplement (e.g., S1801) per 100 ml medium.

8. Mix gently and pour plates.