Baar’s Medium for Sulfate Reducers, Modified with 2.5% NaCl – 250g
Modified Baar’s Medium with 2.5% NaCl is specifically designed for the cultivation of sulfate-reducing bacteria, such as Desulfovibrio and Desulfotomaculum. This formulation includes a higher concentration of NaCl (2.5%) to enhance bacterial growth and activity in saline environments, making it ideal for sulfate reduction studies.
Formulation (Per Liter of Medium):
Sodium Chloride (NaCl): 25.0g
Sodium Citrate (C₆H₅Na₃O₇): 5.0g
Magnesium Sulfate (MgSO₄): 2.0g
Calcium Sulfate (CaSO₄): 1.0g
Ammonium Chloride (NH₄Cl): 1.0g
Yeast Extract: 1.0g
Ferrous Ammonium Sulfate (Fe(NH₄)₂(SO₄)₂): 1.0g
Usage Instructions:
Dissolve 36.0g of Modified Baar’s Medium in 1L of distilled or deionized water, heat and boil until completely dissolved.
Sterilize by autoclaving at 121°C for 15 minutes.
After sterilization, cool the medium to 55°C.
Add 1 vial of 35% Sodium Lactate Solution (contains 0.35g Sodium Lactate and 0.05g Disodium Phosphate) to every 100ml of the medium.
Adjust the pH to 7.5 at 25°C using sterile Sodium Carbonate Solution or Diluted Hydrochloric Acid.
Mix thoroughly and the medium is ready for use.
