B12 Assay Medium

B12 Assay Medium is a microbiological assay medium specifically formulated for the quantitative determination of Vitamin B12 (cyanocobalamin) using suitable test organisms.

The medium supports the growth response of Vitamin B12–dependent microorganisms, allowing accurate measurement of Vitamin B12 content in pharmaceuticals, food products, dietary supplements, and biological samples through turbidity or growth-based assay methods.

⚠ For laboratory research and quality control use only. Not intended for clinical diagnosis or therapeutic use.

🔬 Intended Use

• Microbiological assay of Vitamin B12

• Quality control testing in pharmaceutical industry

• Vitamin analysis in food and dietary supplements

• Research applications in nutrition and biochemistry

📦 Product Specifications

• Product Name: B12 Assay Medium

• Physical Form: Dehydrated powder

• Package Size: 250 g / bottle

• Storage Condition: Room temperature, dry and well-ventilated place

• Shelf Life: 3 years

🧪 Principle of the Method

The assay is based on the growth response of Vitamin B12–requiring microorganisms. The extent of microbial growth is directly proportional to the amount of Vitamin B12 present in the test sample when compared with a standard reference curve.

⚗️ Preparation Instructions

Prepare and use the medium according to the validated assay protocol or relevant pharmacopeial standards.
Sterilization, inoculation, and incubation conditions may vary depending on the test organism and assay method used.

🧫 Storage & Stability

• Store dehydrated medium at room temperature, protected from moisture and light

• Do not use if the medium shows caking, discoloration, or contamination