Azorhizobium Medium Base for Stem & Root Nodule Bacteria Cultivation – 250g

Azorhizobium Medium Base is a specialized microbiological medium designed for the cultivation of stem and root nodule bacteria, including nitrogen-fixing Rhizobium and related species. This medium provides essential nutrients for bacterial growth and supports research in nitrogen fixation, plant-microbe interactions, and agricultural microbiology. For optimal performance, sodium lactate can be added to the medium after sterilization to enhance bacterial growth.

Key Features:

• Ideal for stem and root nodule bacteria cultivation.

• Supports the growth of nitrogen-fixing bacteria for research and agricultural studies.

• High-quality ingredients ensure reproducible and reliable results.

• Sterilization ready: Prepared for autoclaving.

• Long shelf life: Stable for up to 3 years at room temperature.

Instructions for Use:
Dissolve 16.7g of Azorhizobium Medium Base in 1L of distilled or deionized water. Boil until completely dissolved. Sterilize by autoclaving at 121°C for 15 minutes. After sterilization, gently shake the medium to mix and cool to approximately 50°C. Under sterile conditions, add 1g of sodium lactate per 100mL of medium, mix thoroughly, pour into petri dishes, and allow to solidify.

Storage: Store at room temperature, away from direct sunlight. Shelf life: 3 years.

Ingredients per liter:

• Dipotassium Phosphate (K2HPO4): 1.67g

• Dipotassium Hydrogen Phosphate (KH2PO4): 0.87g

• Calcium Chloride (CaCl2): 0.04g

• Ferric Trichloride (FeCl3): 0.004g

• Magnesium Sulfate (MgSO4): 0.1g

• Yeast Extract: 1.0g

• Sodium Chloride (NaCl): 0.05g

• Agar: 13.0g

• pH: 6.9 ± 0.1 at 25°C