ATYP Medium – 250g for Cultivation of Photosynthetic Bacteria
ATYP Medium is specifically formulated for the cultivation of photosynthetic bacteria. It provides all the necessary nutrients and trace elements required for the growth of photosynthetic bacteria, including nitrogen, phosphorous, magnesium, and various vitamins. This medium also contains essential minerals and a variety of growth factors to support optimal bacterial proliferation.
Formulation (Per Liter of Medium):
Ammonium Chloride: 1.0g
Monopotassium Phosphate: 0.5g
Magnesium Chloride: 0.5g
Calcium Chloride: 0.1g
Sodium Chloride: 1.0g
Sodium Acetate: 2.0g
Sodium Succinate: 1.0g
Yeast Extract: 0.5g
Peptone: 0.5g
Ferrous Chloride: 1.8mg
Cobalt Chloride: 0.25mg
Nickel Chloride: 0.01mg
Copper Chloride: 0.01mg
Manganese Chloride: 0.07mg
Zinc Chloride: 0.1mg
Boric Acid: 0.5mg
Sodium Selenite: 0.01mg
Sodium Molybdate: 0.01g
Biotin: 0.01mg
Nicotinic Acid: 0.035mg
Calcium Pantothenate: 0.01mg
Thiamine Hydrochloride: 0.03mg
Vitamin B12: 0.005mg
Pyridoxine Hydrochloride: 0.01mg
Para-Aminobenzoic Acid: 0.02mg
Usage Instructions:
Dissolve 7.1g of ATYP Medium in 1L of distilled or deionized water.
Heat to warm and dissolve completely.
Adjust pH using NaOH or HCl to achieve a pH of 6.8-7.0 after sterilization (at 25°C).
Sterilize by autoclaving at 115°C for 30 minutes.
After sterilization, allow the medium to cool, and store until use.
