APT Broth – Medium for Cultivation of Heterofermentative Lactic Acid Bacteria

APT Broth is a specialized liquid medium designed for the cultivation of heterofermentative lactic acid bacteria and other microorganisms requiring high thiamine concentration. Ideal for food, dairy, and microbiology laboratories, this medium provides essential nutrients and minerals for optimal bacterial growth.

Product Information:

• Name: APT Broth

• Form: Dehydrated powder

• Pack Size: 250g per bottle

• Storage: Room temperature

• Shelf Life: 3 years

Composition (per liter):

• Yeast Extract – 7.5 g

• Pancreatic Digest of Casein – 12.5 g

• Dextrose – 10.0 g

• Sodium Chloride – 5.0 g

• Sodium Citrate – 5.0 g

• Thiamine•HCl – 0.01 g

• Dipotassium Hydrogen Phosphate – 5.0 g

• Manganese Chloride – 0.14 g

• Magnesium Sulfate – 0.8 g

• Ferrous Sulfate – 0.06 g (0.02 g + 0.04 g)

• Tween 80 – 0.2 g

• pH: 6.7 ± 0.2 (25℃)

Preparation Instructions:

1. Dissolve 46.2 g of APT Broth powder in 1 L of distilled or deionized water.

2. Warm gently until fully dissolved.

3. Dispense into containers and sterilize by autoclaving at 121℃ for 15 minutes.

4. Cool and store for use.

5. Ready for cultivation of heterofermentative lactic acid bacteria and other thiamine-requiring strains.