Medium 41 (250g) for microbiological antibiotic potency testing

Antibiotic Medium No. 41 – USP Medium for Microbiological Potency Testing

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Product Overview

Antibiotic Medium No. 41 is a dehydrated culture medium designed for the microbiological determination of antibiotic potency using standardized assay techniques.
The formulation supports reliable microbial growth and assay reproducibility required in pharmaceutical quality control environments.

This medium is manufactured in accordance with USP-referenced formulations and supplied as a stable powder to ensure consistency, ease of preparation, and dependable performance.


Principle of the Method

Microbiological antibiotic assays evaluate the biological activity of antibiotics by measuring the growth response of selected microorganisms under controlled conditions.
The observed response is proportional to the potency of the antibiotic being tested.

Key Features

  • Dehydrated culture medium
  • Designed for microbiological antibiotic potency assays
  • Suitable for routine pharmaceutical testing
  • USP-referenced formulation
  • Consistent performance and reproducibility
  • Easy preparation and extended shelf life

Applications

  • Antibiotic potency determination
  • Microbiological assay procedures
  • Pharmaceutical quality control testing
  • Research and analytical microbiology laboratories

✅ Product Specifications

  • Product Name: Antibiotic Medium No. 41
  • Form: Dehydrated powder
  • Package Size: 250 g
  • Storage Condition: Room temperature
  • Shelf Life: 3 years
  • Reference Standard: USP
  • Final pH: 6.7 ± 0.2 (25°C)

✅ Composition (per liter)

ComponentQuantity
Yeast Extract5.0 g
Pancreatic Digest of Casein9.0 g
Dextrose20.0 g
Monobasic Potassium Phosphate1.0 g
Dibasic Potassium Phosphate1.0 g
Sodium Chloride10.0 g

All ingredients are listed exactly as provided. No functional descriptions added.


✅ Preparation Instructions

  1. Suspend 46 g of the medium in 1,000 mL of distilled or purified water.
  2. Heat with frequent agitation until completely dissolved.
  3. Dispense into suitable containers.
  4. Sterilize by autoclaving at 121°C for 15 minutes.
  5. Cool before use.

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