Medium 4 (Yeast Beef Agar) 250g for microbiological antibiotic potency testing​

Antibiotic Medium No. 4 – Yeast Beef Agar for Antibiotic Potency Testing

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Product Overview

Antibiotic Medium No. 4, also known as Yeast Beef Agar, is a dehydrated solid culture medium developed for the microbiological determination of antibiotic potency.
The formulation contains peptone, yeast extract, beef extract, dextrose, and agar, providing suitable growth conditions for agar-based antibiotic assay methods.

This medium is referenced to USP guidelines and supplied as a powder, offering consistent quality, convenient preparation, and long-term stability for routine microbiology laboratory use.


Key Features

  • Designed for microbiological antibiotic potency testing

  • Solid agar medium suitable for diffusion-based assays

  • Referenced to USP guidelines

  • Dehydrated powder format for easy storage and preparation

  • Reliable and reproducible laboratory performance


Applications

  • Antibiotic potency determination

  • Microbiological assay of antibiotics

  • Quality control testing

  • Laboratory research and analysis

✅ Product Specifications

  • Product Name: Antibiotic Medium No. 4
  • Alternative Name: Yeast Beef Agar
  • Form: Dehydrated powder
  • Package Size: 250 g / bottle
  • Storage Condition: Room temperature
  • Shelf Life: 3 years
  • Reference Standard: USP
  • Final pH (25°C): 6.6 ± 0.1

✅ Composition (per liter)

ComponentQuantity
Peptone6.0 g
Yeast Extract3.0 g
Beef Extract1.5 g
Dextrose1.0 g
Agar15.0 g

✅ Preparation Instructions

  1. Suspend 26.5 g of the product in 1 liter of distilled or deionized water.
  2. Heat to boiling until completely dissolved.
  3. Dispense into suitable containers.
  4. Sterilize at 121°C for 15 minutes or 115°C for 30 minutes.
  5. After sterilization, mix well to prevent agar from settling.
  6. Allow to cool and store for use.

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