Medium 35 (250g) for microbiological antibiotic potency testing

Antibiotic Medium No. 35 – USP Agar Medium for Microbiological Potency Testing

Let us know which dry culture media products you’re interested in, including specifications and quantities. We’ll reply promptly.

Product Overview

Antibiotic Medium No. 35 is a dehydrated agar culture medium designed for the determination of antibiotic potency using microbiological assay techniques.
The formulation provides a stable nutrient base suitable for agar-based microbiological assays and supports consistent and reproducible results.

This medium is referenced to USP standards and supplied as a powdered formulation for convenient preparation and extended shelf life.
It is widely used in pharmaceutical quality control and microbiology laboratories.


Principle of the Method

The assay is based on the microbiological response of test organisms to antibiotics diffused within an agar matrix.
The observed growth or inhibition is related to the concentration or biological activity of the antibiotic under test.

Key Features

  • Dehydrated agar culture medium
  • Designed for antibiotic potency determination
  • Suitable for agar-based microbiological assays
  • USP-referenced formulation
  • Reliable and reproducible performance
  • Easy preparation and long shelf life

Applications

  • Microbiological antibiotic potency testing
  • Agar diffusion bioassays
  • Pharmaceutical quality control laboratories

✅ Product Specifications

  • Product Name: Antibiotic Medium No. 35
  • Form: Dehydrated powder
  • Package Size: 250 g / bottle
  • Storage Condition: Room temperature
  • Shelf Life: 3 years
  • Reference Standard: USP
  • Final pH (25°C): 7.0 ± 0.1

✅ Composition (per liter)

ComponentQuantity
Peptone10.0 g
Glycerol10.0 g
Beef Extract10.0 g
Sodium Chloride3.0 g
Agar17.0 g

All components are fully listed as provided. No functional descriptions added.


✅ Preparation Instructions

  1. Suspend 50.0 g of the product in 1,000 mL of distilled or purified water.
  2. Mix well and heat with frequent agitation until completely dissolved.
  3. Dispense into suitable containers.
  4. Sterilize in an autoclave at 121°C for 15 minutes.
  5. Allow to cool before use.

Review My Order

0

Subtotal