Medium 34 (250g) for antibiotic potency testing with glycerol addition

Antibiotic Medium No. 34 – USP Medium for Microbiological Potency Testing

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Product Overview

Antibiotic Medium No. 34 is a dehydrated culture medium designed for the determination of antibiotic potency using microbiological assay techniques.
The formulation supports consistent microbial growth in potency testing systems and is prepared with glycerol addition during medium preparation, as specified.

This medium is referenced to USP standards and supplied as a stable powder, ensuring ease of preparation, reproducible performance, and suitability for routine quality control testing in pharmaceutical and microbiology laboratories.


Principle of the Method

The assay is based on the microbiological response of test organisms to antibiotics in a defined growth medium.
The degree of growth or inhibition observed is related to the concentration or biological activity of the antibiotic being tested.

Key Features

  • Dehydrated culture medium
  • Designed for antibiotic potency determination
  • Prepared with glycerol addition as specified
  • USP-referenced formulation
  • Consistent performance in microbiological assays
  • Suitable for routine laboratory use

Applications

  • Microbiological antibiotic potency testing
  • Pharmaceutical quality control
  • Laboratory antibiotic bioassays

✅ Product Specifications

  • Product Name: Antibiotic Medium No. 34
  • Form: Dehydrated powder
  • Package Size: 250 g / bottle
  • Storage Condition: Room temperature
  • Shelf Life: 3 years
  • Reference Standard: USP
  • Final pH (25°C): 7.0 ± 0.1

✅ Composition (per liter)

ComponentQuantity
Peptone10.0 g
Glycerol10.0 g
Beef Extract10.0 g
Sodium Chloride3.0 g

All components are fully listed as provided. No functional descriptions added.


✅ Preparation Instructions

  1. Suspend 33.0 g of the product in 1,000 mL of distilled or purified water.
  2. Mix well and heat with frequent agitation until completely dissolved.
  3. Dispense into appropriate containers.
  4. Sterilize in an autoclave at 121°C for 15 minutes.
  5. Allow to cool before use.

Glycerol is included as specified in the formulation.

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