Medium 19 (250g) for microbiological antibiotic potency testing

Antibiotic Medium No. 19 – USP Agar Medium for Microbiological Potency Testing

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Product Overview

Antibiotic Medium No. 19 is a dehydrated agar-based culture medium formulated for the microbiological determination of antibiotic potency.
The formulation provides a balanced nutrient composition suitable for reliable and reproducible agar diffusion–based assay systems.

This medium complies with USP-referenced microbiological assay requirements and is supplied as a stable powder for laboratory preparation.
It is suitable for routine quality control testing in pharmaceutical and microbiology laboratories.


Principle of the Method

The assay is based on the diffusion of antibiotics within an agar medium, where microbial growth response or inhibition correlates with the concentration or biological activity of the antibiotic being tested.

Key Features

  • Dehydrated agar medium
  • Designed for antibiotic potency determination
  • Suitable for agar diffusion assays
  • Referenced to USP standards
  • Consistent batch-to-batch performance
  • Easy preparation and extended shelf life

Applications

  • Microbiological antibiotic potency testing
  • Agar diffusion–based bioassays
  • Pharmaceutical quality control laboratories

✅ Product Specifications

  • Product Name: Antibiotic Medium No. 19
  • Form: Dehydrated powder
  • Package Size: 250 g / bottle
  • Storage Condition: Room temperature
  • Shelf Life: 3 years
  • Reference Standard: USP
  • Final pH (25°C): 5.6 ± 0.1

✅ Composition (per liter)

ComponentQuantity
Peptone9.4 g
Yeast Extract4.7 g
Beef Extract2.4 g
Sodium Chloride10.0 g
Dextrose10.0 g
Agar23.5 g

All components are fully listed as provided. No functional descriptions added.


✅ Preparation Instructions

  1. Suspend 60.0 g of the product in 1,000 mL of distilled or deionized water.
  2. Heat with agitation until completely dissolved.
  3. Dispense into appropriate containers.
  4. Sterilize at 115°C for 30 minutes.
  5. Mix well after sterilization and allow to cool before use.

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